Nature article · 16 tracked changes
PAM-flexible base editing of human T cells…
Abstract
We used a PAM-flexible SpCas9 adenine base editor new base editor and observed a ≥10-fold induction significantly improved knockdown of FOXP3, IL2RA and CTLA4 (n=3, p<0.01).
Main
Adoptive cell therapy is promising, but reliably engineering primary human T cells remains a bottleneck engineering T cells is hard. We profiled three immune-regulatory loci…
Methods
PBMC → CD4+ magnetic selection. RNP electroporation. RT-qPCR; n=3 biological replicates.
9 insertions + 7 deletions — every revision lands as a real w:ins/w:del element with accept/reject markers preserved.
